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The SH1211 genome was subjected to a recombination analysis, and the potential breakpoints with optimal P values based on the χ analysis were identified using the GENECONV method.
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Each marker was tested for recombination events using the GENECONV [ 49], MaxChi [ 50], and RDP methods [ 51].
The sequences were analyzed for gene conversion by using the Geneconv program with default parameters [ 41].
Aligned allergen gene CDS were used for analysis of gene conversion using the GENECONV software as described in Sawyer (1989).
To further establish whether gene conversion has occurred, and identify the boundaries for each species, we made an analysis using the Geneconv program [ 12] (Table 1).
Using the GENECONV program, we also detected that tracts of gene conversions between L and M opsin genes occurred mostly within the intron regions.
The GENECONV method was used to search for putative breakpoints [ 30].
We identified potential gene conversion events within each subfamily using the methods implemented in the GeneConv program [ 51] which identifies identical fragments shared between pairs of nucleotide sequences.
Then, we tested for recombination (and gene conversion) in our datasets by: i) a scan for recombination using the 7 methods implemented in the RDP3 software [ 74] using default settings (i.e. RDP, Bootscanning, GENECONV, MaxChi, Chimaera, SiScan, 3SEQ), ii) the Genetic Algorithm Recombination Detection (GARD) method implemented in the Web interface of HyPhy Datamonkey [ 75].
Using the program GENECONV [ 25] a series of inter- and intra paralog conversion events are evident.
Putative genetic recombination events occurring in Xanthomonas genomes were examined by using the software geneconv [ 86] and PhiPack [ 87].
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