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Gene set analysis was performed using the Gene set enrichment analysis (GSEA) software V2.0.2 (http://www.broad.mit.edu/gsea) (Subramanian et al, 2005).
Enrichment scores for each and every compound in the database were computed using the gene set enrichment analysis algorithm [11].
The clusters of genes identified by CAGED were analyzed using the gene set enrichment analysis in David Bioinformatics resources Huang da et al., 2007 [33] (http://david.abcc.ncifcrf.gov/home.jspand), and Hosack et al., 2003 [34].
Enriched pathways were evaluated using the gene set enrichment analysis of GeneTrail.
First, using the gene set expression comparison tool, 302 pathways (as defined by Biocarta) were analyzed.
Hence, we performed the analysis using the gene set permutation option.
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Using the gene sets approach we identified many prognostic gene sets in breast cancer.
Similar results were observed using the gene sets from KEGG (data not shown).
Using the gene sets defined above, for each gene, we aligned female expression trajectories to male trajectories, and vice versa.
When there are a priori defined gene sets available, the differential coexpression of gene sets can be found by using the gene sets coexpression analysis (GSCA) (Choi and Kendziorski, 2009).
Using the gene sets induced by the enumerations, at this point the stage may be set for exploring the effects and interactions of multiple sets of genes as determinants of the progress and outcome of disease.
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