Sentence examples for using the gene replacement from inspiring English sources

Using the gene replacement approach, we also studied the potentiation of transmitter release by phorbol esters.

The narXL genes were deleted from E. coli strain JCB386 using the gene-replacement method [ 36].

Strains used for the gene replacement experiments: BW25113/pKD20, BW25141/pKD4 and BT340 were obtained from the E. coli Genetic Stock Center CGSCC) at Yale University.

Here, Halomonas TD01 was metabolically engineered using the gene knockout procedure based on markerless gene replacement stimulated by double-strand breaks in the chromosome.

The chromosomal integration vector mini-CTX-lacZ [30], and the gene replacement vector pK18mobsacB were used for reporter construction and gene deletion, respectively.

Prior to electroporation, the gene replacement cassettes of either construct were excised using SwaI restriction digest.

The kanamycin resistance cassette used to construct the flhDC and IS5 gene replacements was removed by expressing FLP recombinase from the helper plasmid (pCP20), after its transformation into the gene replacement mutants [ 27].

The gene replacement was confirmed by PCR assay.

The gene replacement was further confirmed by Southern blot analysis.

Our work reports the creation of a functional in vivo expression reporter for ADGF-A using precise gene replacement homologous recombination procedures.

To investigate the SecG role in adhesion and cell motility we generated SecG deficient cells in strain AX2 using a gene replacement vector in which sequences from position 576 to 2083 of the coding sequence encoding 10 of the predicted 15 ankyrin repeats and the first half of the Sec7 domain (amino acid residues 192 to 695) were replaced by the selection cassette.