Sentence examples for using the gene knockout from inspiring English sources
Here, Halomonas TD01 was metabolically engineered using the gene knockout procedure based on markerless gene replacement stimulated by double-strand breaks in the chromosome.
We conducted our glycerol minimal media tests using the gene knockout essentiality data of Joyce et al.[ 34], an expansion of the study of the Keio collection essentiality to include growth on M9 minimal medium with 1% glycerol.
We validated the E.coli model, and MetaFlux in general, by using the gene knockout component of MetaFlux to simulate the effects of single-gene deletions in E.coli in glucose minimal medium.
A functional glycoproteomic approach could help answer this question by (1) identifying target proteins of these glycosyltransferase genes implicated in cancer, using the glycosyltransferase gene knockout or knockdown cell models and (2) identifying the functional roles of these aberrant glycosylated proteins in cancer cell invasion and metastasis in vivo and in vitro.
We are convinced that the functional analyses using the chitinase gene knockout described in this study provide a much clearer result.
The D5 mutant was constructed using the Targetron gene knockout system (Sigma-Aldrich) to disrupt a region of the hydrogen cyanide biosynthetic operon that encompassed both the hcnB and hcnC genes.
Briefly, the arsC gene from the E. coli DH10B derivative strain, Jude 1, and the E. coli DHB4 strain was replaced with a kanamycin resistance marker derived from plasmid pKD4 using the Wanner gene knockout method.
You used the gene trap Imp1-βgeo knockout mouse, which shows overall growth defects throughout the embryo (and the placenta) by E17.5.
Moreover, when these researchers used the gene editing technology CRISPR to "knockout" the genes in mice, they found that they reduced REM sleep "to an almost undetectable level".
Here we show that abrogation of the cell surface protein E-cadherin, using either gene knockout (Ecad-/) or the neutralising antibody DECMA-1 (EcadAb), allows culture of mouse ES cells as a near-single cell suspension in scalable shake flask culture over prolonged periods without additional media supplements.
Using p21 gene knockout animals, we analyzed the impact of this gene on retinal neuroprotection in the model of light induced retinal degeneration.
