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We used the gene database provided by Biomart (ensembl.org/biomart) using all ENSEMBL genes (version 59) with their untranscripted sequence for human.
ESTs containing SSRs were functionally annotated using the GenBank non-redundant (nr) database and further classified using the gene ontology database.
The differentially expressed proteins were classified using the Gene Ontology database and Kyoto Encyclopedia of Genes and Genomes database.
Second, using the Gene Ontology database to identify cell adhesion-related genes, we obtained 1719 individual probe sets corresponding to genes involved directly or indirectly in cell adhesion.
Proteins in each fraction were categorized as soluble, membrane-associated or membrane using the gene ontology database (http://www.ebi.ac.uk/GOA/).ac.uk/GOA/
Based on the approach used by Wu and Zhang [26], we also searched a set of genes related with dental development using the Gene Ontology database (browser AmiGO - http://amigo.geneontology.org/cgi-bin/amigo/browse.cgi [54]).
For BYPASS predictions, the GO terms were assigned to the predictions using the Gene Ontology database directly.
Further, we expanded the expression analysis of AP2/ERF genes using the gene expression database SRA (ERA047687) submitted by Brown et al [ 34].
Functional integration of gene expression, i.e., identification of gene networks, was performed using the 'Gene Ontology' database AmiGO (http://amigo.geneontology.org).org
We evaluated the clustering performance based on its ability to produce biologically meaningful clusters using the Gene Ontology database as a common reference [ 89, 90].
Analysis using the Gene Ontology database through the Gather web tool suggested that several transcripts regulated by DMXAA in stromal cells encoded molecules associated with defence/immune responses.
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