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In this study, eight coding sequences (CDS) of AP2 gene were selected from different plant species using the GenBank database.
Primers to amply the 16 S rRNA from several bacteria of the Firmicutes phylum (Listeria monocytogenes, Staphylococcus epidermidis, S. aureus, Enterococcus faecalis, Lactobacillus casei, Streptococcus pyogenes, Clostridium perfringens and Bacillus cereus) were designed using the Genbank database and were aligned using ClustalW.
Web-based similarity searches using the GenBank database were used to determine the homologous degrees of zlaI and zlbI protein sequences to those of other AHL synthases.
ODN sequences were subjected to a BLAST search on the National Center for Biotechnology Information BLAST server using the Genbank database.
Sequencing results were analyzed using the BioEdit sequence alignment editor (Ibis Biosciences); primer sequences were removed and nucleotide similarity comparisons were made using the GenBank database.
The nucleotide sequences were identified with a BLAST search (http://www.ncbi.nlm.nih.gov/BLAST/) using the GenBank database.
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As a source of mt-12S rRNA variations we used the GenBank database.
Analysis of results was carried out using the GenBank nucleotide database and BLAST algorithm [39].
Of the remaining 10 SNPs, five were verified electronically using the GenBank SNP database (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi db = Snp).
DNA fragments from the bands were sequenced and each OTU sequence and each sequence from the DGGE bands were assigned to taxonomic groups by BLAST using the GenBank nucleotide database as described above.
Primers for qPCR were designed by using the GenBank nucleotide database and the online software available from Genscript (www.Genscript.com).
More suggestions(15)
using the MicrobesOnline database
using the Aria database
using the genbank nr
using the Cassandra database
using the genbank host
using the PostgreSQL database
using the Mascot database
using the Webribo database
using the genbank entry
using the genbank data
using the genbank accession
using the genbank gene
using the Amadeus database
using the SortMeRNA database
using the ProfCom database
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