Sentence examples for using the gateway method from inspiring English sources

In our hands, using the Gateway method of cloning and the commercially available nematode ORFeome library we obtained a 59% success rate.

Expression plasmids were produced using the Gateway method.

Tmub1/HOPS was cloned using the Gateway™ system.

Lentiviral vector for SFRS3 protein overexpression was constructed using the Gateway pENTR11 and pLenti6.2/V5-DEST Gateway vectors from Invitrogen.

The LRRK2 constructs were made using the Gateway system as described previously [30].

LRRK2 (wild-type, G2019S, R1441C) was then introduced into the vector using the Gateway clonase (Invitrogen).

Lentiviral vector plasmids were constructed by recombination using the Gateway system (Invitrogen, Carlsbad, CA, USA).

Finally, we use the gateway nodes identified by our method as well as functional databases and literature to propose new targets for study of aging in the mouse hippocampus.

Drosophila iRhom (Q76NQ1, cDNA HL06695) was cloned into pUAST vector (pTW, The Drosophila Gateway vector collection, http://www.ciwemb.edu/labs/murphy/Gateway%20vectors.html) using the Gateway system (Invitrogen).

We constructed a total of 15 cDNA libraries (Table 1) using the SMART™ method and the engineered Gateway-based pENTR- SfiI cloning vector.

Baits were tagged with GST using the Invitrogen Gateway system as previously described (15, 51).