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Since neither our approach nor that of using the full coding sequence can be validated experimentally, it is unclear which yields more accurate results.
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Phylogenetic analysis using the full Vtg coding sequences was used to examine the evolution of Vtg genes among mosquitoes.
Only the informative alignments obtained using the full rainbow trout coding sequence (CDS) or a substantial part of the CDS are presented.
Sense and anti-sense RNA probes were generated using the full-length coding sequence for FGF-4 cloned into Bluescript (Stratagene, La Jolla, California) [ 24], forming a template for a MAXIScript in-vitro transcription (Ambion, Austin, TX) using a [P] UTP label.
It addresses a simplified spherical configuration while using the full three-dimensional code.
For in vitro transcription the entire insert was used containing the full coding sequence of the given molecule indicated above.
To synthesize cRNA probes from non-mammalian vertebrates the entire cDNA insert was used containing the full coding sequence of Dr prominin-1a (HQ386793), Am prominin-1 (DQ285041) and Gg prominin-1 (HQ386789), except for prominin-1b (nt 234 1,599; AF373869).
A large publicly available listing of 567,921 different medical phrases were automatically coded using the full UMLS metatathesaurus and the Category 0 vocabularies.
In some cases 5'-RACE was used to determine the full coding sequence from total RNA isolated from spleen tissues.
A homologous candidate gene approach was used to generate the full coding DNA sequences (CDSs) of SRK and full sequences with the 5′ promoter regions of SCR in 11 SC B. napus accessions based on the CDSs of BnS-7 (BrS-29) on the A genome and BnS-6 (BoS-15) on the C genome.
This algorithm allows specifying the maximum count of mismatched positions between each primer and the target sequence, and to use the full IUPAC code (e.g., R for purines or Y for pyrimidines).
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