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The absolute hand of Drosophila ORC was determined using the Freehand test (Rosenthal and Henderson, 2003; Henderson et al., 2011).
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The final refined Drosophila ORC EM volume shown in Figure 1 figure supplement 2C was used in the freehand test using 0° and +50° tilt-pair images.
Total and humanized areas were determined by histological examination, measured using the freehand outline tool, and calculated using Axiovision V4.8 software.
The silicon nanocrystals were marked using the freehand selection tool in ImageJ [17].
All the bacterial cells in the images were selected individually using the freehand selection tool of the software.
(a) Data acquisition using the freehand SPECT device; radioactivity is measured with the probe from multiple directions.
Filament contours were traced using the "freehand line tool" and exported as XY-coordinate sets.
Amylase-positive area/pancreas area was measured manually with ImageJ using the freehand selection tool.
Epicardial and endocardial borders were outlined using the freehand drawing function of ImageJ (National Institutes of Health, USA).
The total lesion area was determined using the freehand drawing tool.
16 17 SC was traced using the freehand tool in Fiji, and area calculated automatically (figure 1).
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