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FRAP was measured using FRAP profiler on average projections of confocal stacks, using the freehand selection tool to select the bleached area and normalized to total cellular fluorescence.
To obtain values for the SCV-associated marker in these cells, the bacterial cluster in the best focal plane was selected using the freehand selection tool and the software was then allowed to extend the selection through all z-sections in both channels.
All the bacterial cells in the images were selected individually using the freehand selection tool of the software.
The silicon nanocrystals were marked using the freehand selection tool in ImageJ [17].
For each experimental condition, we traced the outline of the rod spherule, horizontal axonal endings and bipolar cell dendrite using the freehand selection tool (green, Fig. 1A).
Amylase-positive area/pancreas area was measured manually with ImageJ using the freehand selection tool.
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To analyze the area of interest manually, we used the "freehand selection" tool to enclose the fluorescent area.
2. We used the "freehand selection" tool to draw a line around a single cell we wanted to measure and then selected an appropriate measure in the "analyzis" tool; in all cases, we analyzed many cells (20 30) in each picture.
Formalin-fixed tissue sections were stained with hematoxylin and eosin (H&E) using standard staining protocols and analyzed using the freehand line selection tool of Image J (Rasband, ImageJ, NIH, Bethesda, Maryland; http://rsb.info.nih.gov/ij/).
(a) Data acquisition using the freehand SPECT device; radioactivity is measured with the probe from multiple directions.
Filament contours were traced using the "freehand line tool" and exported as XY-coordinate sets.
More suggestions(15)
using the freehand region
using the freehand outline
using the patient selection
using the covariate selection
using the freehand tool
using the color selection
using the elimination selection
using the freehand line
using the freehand freehand
using the freehand uniform
using the freehand software
using the ortholog selection
using the Kish selection
using the freehand machine
using the path selection
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