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A mathematical method for assessing whole S. bicolor stalks using the fourth internode from the base of the plant proved to be an effective high-throughput strategy for assessing stalk biomass, soluble sugar concentrations, and cell wall composition and allowed calculation of total stalk fermentable sugars.
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A new high-throughput methodology was developed for screening large variable populations of S. bicolor for biomass, soluble sugar concentrations, and cell wall composition using a volumetric correlation between the fourth internode from the base of the stalk that had expanded more than 2 cm, to that of the whole stalk.
Whilst not all ratios approached 100%, all had small standard errors suggesting that these fixed ratios were consistent across genotypes, and could therefore be used in extrapolating measurements from the fourth internode to whole stalks.
Calculations using V ratio and measurements from the fourth internode were compared to those taken from the whole stalk.
In our case, the fourth internode from the base of the stalk was used as the sample internode since a stable relationship between sugar concentrations in the fourth internode and the whole stalk had been repeatedly observed in previous field measurements (unpublished data) and it has been shown that lower internodes in maize stalks within a genotype are less variable [ 24].
Fig. 3 Cell wall material density of the fourth internode in 2015.
a flexural rigidity, b secondary moment inertia, and c Young's modulus of the fourth internode.
a bending moment at breaking, b section modulus, and c bending stress of the fourth internode.
The fourth internode above ground level was collected from 12 plants for biochemical analyses.
During harvest of the fourth internode four field measurements were taken: stalk height (H), stalk base diameter (R), stalk top diameter (r), and length from the base of stalk to the base of the fourth internode (H B ).
The length of the second and the fourth internodes of A1 were 55 and 21%% shorter than that of A9, respectively.
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