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Cell size measurements were accomplished using the forward light scatter (FSC) data of the FACS.
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To investigate the scattering energy from the Ag NRA, an integrating sphere with a diameter of 5 cm is used to measure the forward light scattering.
Cells were counted with a fluorescence-activated cell sorting (FACS) Calibur flow cytometer and CellQuest Pro software (BD Biosciences, Mountain View, CA, USA) and single cells were gated away from clumped cells by using forward light scattering on an FL2-width versus FL2-area dot plot.
A PMT voltage of 415 V was used for forward light scatter and 590 V used for emission between 510 and 580 nm (gain = 1).
To determine whether an unmodified commercial wavefront aberrometer (irx3) can be used to estimate forward light scattering and how this assessment matches estimations obtained from the C-Quant straylight meter.
Heterozygous Tg[gSAGFF202A] was genotyped using the primers: Forward: 5′-GCTCAAGTGCTCCAAAGAAA-3′; Reverse: 5′-ATCAGCAGGCAGCATGTCC-3′. Heterozygous and homozygous Tg[gSAGFF202A SILL mCherry] were screened with a stereomicroscope under ultraviolet light.
Using the lighter, activate the paraffin cubes.
Always mix using the lighter color first.
Use the red light, green light method.
Use the black light and have fun.
We analyzed the relative cell size by flow cytometry using forward angle light scatter.
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