Sentence examples for using the formula efficiency from inspiring English sources
Using the formula (Efficiency = 10(−1/Slopefficiencyiciency was calculated.
Real-time PCR efficiencies for each reaction were calculated using the formula: Efficiency (E) = [10(1/ slope )] – 1, from the slope values given by Rotor-Gene software.
PCR efficiency was calculated by converting the slope produced by the linear regression of the curves to percentage efficiency using the formula: Efficiency = −1 + 10 (−10slope).
Real-time PCR efficiencies for each reaction were calculated using the formula: Efficiency (E) = [10(1/ slope )] - 1, from the slope values given in the GeneAmp 5700 Sequence Detection System.
Correlation coefficients between Ct and DNA template concentration for each TaqMan assay were calculated by regression analysis and amplification efficiency was calculated using the formula: efficiency = -1 + 10^ -1/slope).
The estimates of amplification efficiencies were calculated for each of the 12 genes using the formula Efficiency = 2 − 1 / slope − 1 where slope was estimated from the regression of CT measurements versus RNA concentration.
Ct values were converted in qBase software v1.3.5 [ 39] into non-normalized relative quantities, corrected by PCR efficiency, using the formula Q = EΔCt where E is the efficiency of the gene amplification and ΔCt is the sample with the lowest expression in the data set minus the Ct value of the sample in question.
Cq values were converted in qBase software v1.3.5 [ 35] into non-normalized relative quantities, corrected by PCR efficiency, using the formula Q = EΔCq where E is the efficiency of the gene amplification and ΔCq is the sample with the lowest expression in the data set minus the Cq value of the sample in question.
Ct values were adjusted for starting concentration and reaction efficiency using the formula: 40 - (Sample Mean Ct Target + (Median 28S - Sample Mean 28S) * (Slope Target/Slope 28S)).
The purified PCR products were cloned in pGEM-T easy vector system and the plasmid DNA was used to generate calibration curves, which also permitted evaluation of PCR efficiencies using the formula E = 10[-1/slope].
The PCR efficiencies were calculated from standard curves using the formula E=10[−1/slope] where E is the efficiency and slope is the slope of the standard curve.
