Given a hypothetical peak temperature of 50°, we sought to determine over how large a region the laser-induced temperature exceeded 35°, a common temperature used for whole-body heat shock in worms, using the following three illumination modes: CW illumination; pulsed infrared (PIR) illumination; and pulsed illumination with objective cooling.
Based on these considerations, we have chosen to use the following three modes: MODE 1: nodes in MODE 1 do not proactively announce their location through the propagation of distance vectors.
Co., Tokyo, Japan) images of the maxillary and mandibular dentoalveolar regions were acquired for diagnosis and treatment planning, using the following settings: normal mode (16.8 s, 4.10 mGy, 90 kV, and 4 mA); slice thickness, 0.147 mm; field of view (FOV), 81 × 74 mm; and voxel size, 0.146 mm.
Using the plugin "bunwarpj" [ 17] both images were registered using the following parameters: registration mode: "mono"; initial deformation: "fine", final deformation: "super fine", landmark weight =1, image weight =0.5, Verbose = yes.
Sequencing was simulated with Wgsim from the Samtools package (Li et al., 2009) using the following parameters: paired-end mode with 2 × 100 bp reads, an insert size of 300 bp (std = 50) and a base error rate of 0.01.
Parametric multipoint analysis was performed with MERLIN v1.0-alpha (Ann Arbor, MI), using the following parameters: autosomal-dominant mode of inheritance, gene frequency of 0.001, 4 age-dependent liability classes, and 4 age-dependent phenocopy rates (0.00, 0.01, 0.03, and 0.05 corresponding to liability classes 1 4, respectively).
MS peptide profiling was performed using the following instrument settings: reflector mode, positive voltage polarity, pulsed ion extraction delay 20 ns, ion source voltage "1" 25 kV, ion source voltage "2" 21.7 kV, reflector voltage "1" 26.3 kV, reflector voltage "2" 13.8 kV, reflector detection voltage 1.732 kV, sample rate 0.5 GS/s.
Building on the above analytic analysis, timing offset t ϕ can be estimated in NDA mode using the following optimization t ̂ ϕ = arg max τ ∈ 0, T s J , where J = ∫ 0 T I x ̄ b ( t, τ ) 2 dt.
Quantitation was performed on a QExactive mass spectrometer (Thermo Scientific, San Jose, CA, USA) equipped with a APCI source operating in the negative ionization mode, using the following parameters: sheath gas flow: 35 arbitrary units; auxiliary gas flow: 10 arbitrary units; discharge voltage: 5 v; capillary temperature: 300°C; S-lens RF level: 90; vaporizer temperature: 350°C.
