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After working through all five vignettes, participants reported the prevalence of each of the diagnoses2 in Table 1 using the following instructions: "For each diagnosis, please rate how often patients on the general medicine service have that diagnosis.
In one experimental condition, participants were asked to worry, using the following instructions on the screen: Now we would like you to worry about the topic that you chose earlier in the experiment.
In the other condition, participants were asked to engage in mental arithmetic, using the following instructions on the screen: For a short period, we would like you to do a mental subtraction in your head.
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Use the following instructions to do so: 3211 = 0. 2221 = 1.
Once you've reached the home page at Imgur.com, use the following instructions to upload your file: Click "Upload Images" at the top of the page.
So, use the following instructions as a gauge for determining how and when you should wash and care for your products.
The RNA was transcribed using MEGAscript High Yield Transcription Kit following instructions provided by the manufacturer (Ambion).
DNA was extracted using the Qiagen DNeasy Blood & Tissue Kit following manufacturer instructions.
The mutagenesis protocol was performed using the manufacturer instructions and the following primers: C169Yfor 5′-GCAGCGGCTGGTGAAGTACAACGCCTGGCCTTG-3′ C169Y rew 5′-CAAGGCCAGGCGTTGTACTTCACCAGCCGCTGC-3′.
RNA was isolated using the QIAGEN RNeasy Midi Kit, following the manufacturers' instructions.
The concentration of the purified Bam complex was then determined using the Bio-Rad DC Prothen Assay following the manufacturer's instructions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com