Two identically designed onsite experiments were conducted using the following filter systems: (a) a vertical flow (VF) peat filter, (b) a vertical flow peat/ash sediment filter (both materials in equal volumes) followed by a horizontal flow (HF) peat filter.
Finally, the samples were observed under a fluorescence microscope (Leica DMI 4000B, Leica Microsystèmes SAS, NANTERRE, France) using the following filter combination: UV/violet excitation band at 2.92 to 3.5 eV with an observation spectral range of < 2.64 eV.
In both agonist and antagonist modes, following 1 to 2 hour incubation at room temperature, the 520/490 TR-FRET ratio was measured with a PerkinElmer Envision fluorescent plate reader with TRF laser excitation using the following filter set: excitation 330 nm, emission 495 nm, and emission 520 nm.
Sections were examined in an Axioplan microscope (Carl Zeiss) equipped with both an epifluorescence system, using the following filter combination: BP450-490, FT510 and LP520.
Cellmask fluorescence (Ex 649, Em 666) was detected using the following filter set: excitation filter BP 575 625, beam splitter 645 and emission filter 660 710.
EGFP, DsRed2 and DAPI were imaged using the following filter from AHF-Analysetechnik (Tübingen, Germany): DsRed/GFP (F51-019), DsRed (F46-005), endowGFP (F41-017), DAPI/GFP (F51-012).
For each method A, B, and C the absolute number of differentially expressed genes was identified using the following filtering strategy: (i) filtering by present calls, followed by (ii) filtering by fold-change, and (iii) filtering by false discovery rate (FDR).
Therefore, we identified these sequences by using the following filtering criterion: O% < 90% with the chloroplast genome and O %≥ 99% with the nuclear genome together with the ID% set to ≥99% to both genomes.
We looked for reads exhibiting this alignment pattern in the RI japonica data set using the following filtering criterion: O% < 80% with the chloroplast and nuclear genomes and ID% > 99% with both genomes (further details in Fig. 2).
Given an image, A, to extract edges, we used the following filter kernels provided by [24].
For GCaMP3.0, Arclight, and SpH imaging, we used the following filter set (Chroma Technology, Bellows Falls, VT): excitation, HQ470/x40; dichroic, Q495LP; emission, HQ525/50m.
