PCR analyses were carried out using the following cycle parameters: 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec, and 60°C for 1 min. Fluorescence was quantified, and analyses of amplification plots were performed with the 7300 Real Time PCR System (Applied Biosystems).
RTQ-PCR was performed using the following cycle parameters: 3 minutes at 95°C, followed by 40 cycles of 15 sec at 95°C and 40s at 60°C.
The PCR was performed in a commercial real-time thermocycler (ABI Prism 7000, Applied Biosystems, Germany) using the following cycle parameters: a 94°C predenaturation for 3 min, followed by 35 cycles of 94°C for 40 seconds, 56°C for 50 seconds, and 72°C for 90 seconds, and an extension cycle of 70°C for 10 min.
PCR was performed under linear conditions using the following cycle profile: initial incubation (15 min at 95°C); followed by 30 cycles of annealing (45 s at 60°C), extension (45 s at 72°C) and denaturation (60 s at 94°C); and terminating with 15 min at 72°C.
The reactions were performed using the following cycle conditions, an initial 94°C for 2 min, followed by 30 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and the final 5 min extension at 72°C.
Reactions were analysed on an ABI 7000 real-time PCR machine using the following cycle conditions; 2 minutes at 50°C, 10 minutes at 95°C, followed by 45 cycles of 15 seconds at 95°C and 60 seconds at 60°C.
PCR was performed using the following cycling parameters: one cycle of 2 min at 96 °C, 35 cycles of 30 s at 96 °C, 30 s at 60 °C and 1 min at 72 °C, and a final extension of 10 min at 72 °C.
The PCR reactions were performed using an ABI 7900HT Fast Real-Time System (Applied Biosystems) using the following cycling protocols: 40 cycles of 15 s at 95°C and 60 s at 60°C.
GAPDH was amplified using the following cycling parameters: 22 cycles of 30 s at 95°C, 30 s at 56°C, and 30 s at 72°C.
All reactions were carried out using the following cycling parameters: 30 cycles of 94°C for 1 min, annealing temperature ranging from 54 to 59°C for 1 min, and 72°C for 1 min for 30 cycles.
PCR was performed on 50 100 ng of extracted DNA using the following cycling conditions: 35 cycles at 94°C (30 s), Tm at 62°C (30 s) and 72°C (1 min), and 10% DMSO (dimethylsulphoxide).
