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Following a midline incision to expose the bregma, four burr holes were drilled bilaterally using the following coordinates with reference to the bregma, site 1 and 2: right and left, −1.8 mm; lateral,±1.1 mm; dorsal to ventral, 2.4 mm; site 3 and 4: right and left, −2.6 mm; lateral,±1.6 mm; dorsal to ventral, 2.7 mm.
To establish intracranial tumors GL261 cells (125,000 cells) were implanted unilaterally into the right hemisphere using the following coordinates from Bregma: posterior −1.06 mm; lateral 3 mm and a depth of 3 mm, according to the atlas of Paxinos and Franklin [51].
The lateral CC was marked using the following coordinates relative to Bregma: anterior posterior −2, medial lateral +0.5, and dorsal ventral −1.2 [ 44].
Samples (brain homogenates and PBS) were injected into the right hippocampus using the following coordinates as measured from bregma: antero-posterior (AP), -1.8 mm; medio-lateral (ML), -1.8 mm; dorso-ventral (DV), -1.8 mm.
The electrode was inserted through the skull, which is still very soft at day P4 using the following coordinates from bregma: AP = −1 mm, L = ±0.5, and P = −3.5 mm.
Cells transplantation into adult mouse brains were performed as previously described in [ 25] with the help of a stereotaxic table using the following coordinates from the Bregma: AP, 1.0 mm; L, 1.5 mm left and 2.5 mm below the skull surface.
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For injection of the AAV8-cmv-MitoDsRed in the SNpc of 10-month old mice, we used the following coordinates: antero-posterior (AP) -2.9 mm and mediolateral (ML) 1.6 mm relative to bregma, dorsolateral (DV) -4.9 mm relative to skull surface.
The bone was then perforated to expose the meninges and 4 μl of a suspension of A25T aggregates was injected at a flow rate of 1 μl/10 min using the following stereotaxic coordinates: AP=−0.22 mm; L=−1.0 mm left; and DV=−2.0 mm.
29 Rigid-flexible molecular docking was applied to the center of the heme-associated binding cavity using the following Cartesian coordinates: x =−15.67 Å, y =−22.49 Å, and z =−11.2 Å.
The mice were anesthetized, and Aβ(25 35) (25 nmol in 5 μl) or the reverse non-active sequence, Aβ 35 25) (25 nmol in 5 μl), was injected into the right ventricle using the following stereotaxic coordinates from the bregma (mm): A -0.22, L -1.0, and V 2.5.
A brainstem slice of 2 2.5 mm was cut throughout the medulla containing the NTS region and using the following stereotaxic coordinates: 3 3.6 mm caudal and 1 1.5 mm lateral to lambda [ 26]; the right and left NTS from the same rat were isolated under microscope (M650, Wild Heerbrugg, Switzerland) and pooled.
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