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In brief, total lipids in the serum were extracted using the Folch procedure and FAs were then methylated with BF3/methanol.
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Lipids were extracted from an aliquot of cell suspension using the Folch method [27].
Total lipids were extracted from 1 ml aliquots of FBS and FBSLess using the Folch method modified by Hernell [20], [21].
Lipids from livers were extracted using the Folch method [ 28].
Sebutapes were subsequently extracted by using the Folch method.
Lipids were extracted from fresh liver tissue using the Folch method [ 15].
After killing the Ahr /– mice, 50 mg of liver was extracted using the Folch method (Folch et al. 1957), lyophylized, and reconstituted in 20 μL of DMSO; 0.1 μL of the DMSO-reconstituted extracted was used for the reporter assay.
Liver and skeletal muscle TG content were measured using the Folch method for lipid extraction followed by spectrophotometric measurement of TG content (Thermo Electron, Louisville, CO) as previously described (Cummings et al., 2010b; Folch et al., 1957).
Lipids were extracted from feces using the Folch extraction method and radioactivity was determined by liquid scintillation counting.
Quantification revealed fewer (p < 0.01) MCFAs when extracted using the Folch method than when detected by direct transmethylation: 3.5 % compared to 6.7%%, respectively (Figs. 1A and C).
Stool samples were analyzed for fat using the Folch method [ 22], and study formula samples were analyzed by the method of Bligh and Dyer [ 23].
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