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Lipids were extracted from an aliquot of cell suspension using the Folch method [27].
Total lipids were extracted from 1 ml aliquots of FBS and FBSLess using the Folch method modified by Hernell [20], [21].
Lipids from livers were extracted using the Folch method [ 28].
Sebutapes were subsequently extracted by using the Folch method.
Lipids were extracted from fresh liver tissue using the Folch method [ 15].
Quantification revealed fewer (p < 0.01) MCFAs when extracted using the Folch method than when detected by direct transmethylation: 3.5 % compared to 6.7%%, respectively (Figs. 1A and C).
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Lipids were extracted from feces using the Folch extraction method and radioactivity was determined by liquid scintillation counting.
In brief, total lipids in the serum were extracted using the Folch procedure and FAs were then methylated with BF3/methanol.
Unless otherwise specified, lipid extraction was performed using the modified Folch method [ 47].
Lipid extraction from dried tissue sections on glass slides was performed using the modified Folch method as described previously.
For the measurement of the lipid content in cells, the Folch method was used.
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