Sentence examples for using the fluorometric substrate from inspiring English sources

To further quantify the GUS staining results in Figure 3C, GUS activity was measured using the fluorometric substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) [51].

Resistance mediated by efflux proteins and mutated topoisomerase II was evaluated using the fluorometric microculture cytotoxicity assay (FMCA) [41].

Caspase-3 activity was measured in retinal protein homogenates using the fluorometric CaspACE assay system (Promega).

Moreover, even when using the fluorometric BACTEC technique, the results of mycobacterial culture still take one to two weeks [ 22].

Activation of caspases was measured using the fluorometric assay kit (Calbiochem), according to the manufacturer's protocol.

RNA concentration was determined using the fluorometric Qubit 1.0 by Quant-iT RNA BR Assay Kit (Life Technologies, Carlsbad, California, USA).

Cathepsin B activity was measured using the synthetic fluorometric substrate Z-Arg-Arg-NHMec as previously described [ 10].

To investigate whether caspase-3 is involved in this pathway, cells cultured with rGal-1 for 16 h were lysed and caspase-3 activity was assessed using the specific fluorometric substrate Ac-DEVD-AFC (N-acetyl-Asp-Glu-Val-Asp-AFC 7-amino-4-trifluoromethyl coumarin).

Caspase activity was monitored by measured the degradation of the fluorometric substrate Ac-DEVD-AMC as previously described [ 16], using the Assay kit (Promega Corporation, Madison, WI, USA).

The fluorometric substrate Ac-DEVD-AMC and Ac-YVAD-AMC were used for the determination of caspase-3 and caspase-1&4 activities.

Caspase-3 activity was determined by the cleavage of the fluorometric substrate z-DEVD-AMC (Upstate Biotechnology, Lake Placid, USA) according to the manufacturer's instructions.