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Transformants with ectopically integrated ku70 deletion constructs were therefore identified using the fluorescent signal of GFP.
Fluorescent signals in Figure 2Q' , Figure 4C and Figure 4C' were obtained using the fluorescent signal emitted from NBT/BCIP precipitate, an alternate method to reflection microscopy.
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We have made no attempt to map the tumor within the ear using the fluorescent signals, but this is an obvious future direction, and one that will require significant software application.
We used the fluorescent signal peak ratio as an indicator of the copy number ratio of the two alleles.
We used the fluorescent signal from label-free collagen fibers interacting with a single AuNP to map the collagen structure.
These two "degraded" systems use the fluorescent signal in very different ways and comparing their ability to localize the depth of the fluorescent inclusion is of interest.
For all substrates used, the fluorescent signal was higher in the treated biofilms compared to the untreated biofilms, confirming the increased extracellular lipase activity for the treated biofilms.
The localization of Ca2+ ions in anther tissues in planta and in tetrads, microspores and pollen was monitored in hand-cut anther sections using the fluorescent yellow signal of the chlorotetracycline (CTC)–Ca2+ complex, according to the procedure standardized by Falasca et al. (2008).
SYBR Green I real-time PCR relies on detection of a fluorescent signal using the fluorescent chimeric dye SYBR Green I, eliminating the need for a specific probe.
The main problem in cell imaging using the fluorescent nanoprobes is that the fluorescence signal is easily affected by the background noises caused by the cells, matrix, and the nonspecific scattering lights.
The reaction was stopped after 7 min at RT (20 °C) using standard procedure and the fluorescent signal was measured using the standard procedure described previously [24,31,39,40].
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