Sentence examples for using the fluorescent probes from inspiring English sources

Generation of nitric oxide (NO) and superoxide (O2−*) was assessed using the fluorescent probes diaminofluorescein (DAF-2DA) and dihydroethidium (DHE, both invitrogen), respectively, by fluorescence microscopy or analysis by the IN Cell Analyzer (GE Healthcare).

MNC migrating towards BK (BKmig) were assessed for capacity to support endothelial cell function in a matrigel assay, as well as generation of nitric oxide (NO) and superoxide (O2−*) by using the fluorescent probes diaminofluorescein and dihydroethidium.

Generation of ROS and RNS was assessed by the measurement of superoxide (O2−), hydrogen peroxide (H2O2), and peroxynitrite (ONOO−) generation using the fluorescent probes DHE [47], DCFH-DA [48] and DHR-123 [49], respectively.

The intracellular accumulation of ROS was determined using the fluorescent probes 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA).

Intracellular accumulation of ROS was determined using the fluorescent probes 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, Sigma-Aldrich).

Experiments using the fluorescent probes H2DCFDA, rhodamine 123, Hoechst 33342, and negative controls support the hypothesis of clusters of mitochondria at polyp-stolon junctions.

In three independent experiments, each performed with triplicate samples, ROS levels were determined using the fluorescent probe DCFDA and the fluorescence of cells was quantified by flow cytometry.

Additionally, the action of casearins on parasite membranes was investigated using the fluorescent probe SYTOX Green.

Using the fluorescent probe SYTOX Green suggested that the alkenylphenol disrupted the Leishmania plasma membrane upon initial incubation.

To investigate the mechanisms enhancing extracellular expression, the permeability of the E. coli outer membrane was assessed using the fluorescent probe NPN (Fig. 3a).

The fabrication technique of using the fluorescent probe of PDI-HIS, copper ion, and graphene oxide (GO) which enhances the selectivity and sensitivity for detecting PPi, a cancer biomarker (Muthuraj et al. 2015).