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The efficacy of re-cellularization was determined by using the fluorescent marker CellTracker CMFDA and anti-CD31 immunostaining.
Mitochondrial membrane potential (Δψm), was evaluated using the fluorescent marker tetramethylrhodamine ethyl ester TMRE (Sigma) as previously described [27].
The cultures were tested for confluence and transepithelial leakage using the fluorescent marker calcein, as described in the Methods.
The effect of DDAH I on tumour perfusion was examined using the fluorescent marker Hoechst 33342 (Smith et al, 1988).
We next assessed neuronal damage in the brain using the fluorescent marker Fluoro-Jade B (FJB), which selectively labels injured neurons [ 36, 37].
The presence of the hyperexpression Her-2/neu in cases of "++" reaction is conducted with the help of hybridization method in situ using the fluorescent marker FISH (fluorescent in situ hybridization).
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In this study, we applied and compared a feasible method, using the fluorescent markers propidium iodide and Sytox to evaluate the viability of C. elegans larvae after drug treatments.
Here, we have used the fluorescent marker DsRed [ 14, 22] and the promoter of the Pdf2.1 gene [ 20] from Arabidopsis because we were especially interested in overexpressing genes that are downregulated in syncytia induced by the beet cyst nematode H. schachtii in Arabidopsis roots [ 23].
ROS levels were measured using the MitoSOX fluorescent marker (Invitrogen), which selectively reacts with superoxide.
Replacement of an ORF with GFP, as we report here for the gtbp-1 locus, is an effective method to generate marked null alleles that can be maintained using the linked fluorescent marker.
The BBB's permeability was assessed by using this fluorescent marker following a two-step process.
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