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Simulations were performed using the fitness model.
Using the fitness model, we found that in facultative cooperation, small fitness benefits generated less intermixing than large fitness benefits.
(B ) In simulations using the fitness model, facultative cooperation conferring smaller fitness benefits required greater community heights to exhibit a significant level of intermixing.
Finally, using the fitness model, we tested intermixing in facultative cooperation by varying the relative magnitude of cooperative benefits compared to the basal fitness of the two interacting populations.
To make this point more explicit, we have added to the main text: "Using the fitness model, we found that in facultative cooperation, small fitness benefits generated less intermixing than large fitness benefits.
In most simulations using the fitness model, the basal fitnesses of both partners were non-zero, and therefore ↑ represented strong facultative interactions: a participant could survive on its own at its basal fitness but fared much better in the presence of its partner.
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To test whether intermixing between cooperators was affected by other members of a community, we used the fitness model to simulate communities composed of five interacting species.
Finally, we used the fitness model to show 'strongly cooperating partners intermix' could be generalized to communities consisting of multiple species.
Parameter values used in the fitness model.
Parameter values used in the fitness model in Figure 3B.
We also predicted successful progenitor strains using the fitness inference based on the SBD model which yields results very similar to the ranking by LBI sometimes slightly better, sometimes worse depending on parameter choice.
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