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The sequential ChIP studies were performed as previously reported [10] and following the sequential immunoprecipitation studies described, using the first primary antibody (anti-EGFR) and the second primary antibody (anti-Src).
We identified all incident female colon cancer patients from 1996 to 2002 using the first primary cancer site code 15-233, cancercancer) (n = 49,086) in the SEER Patient Entitlement and Diagnosis Summary File (PEDSF).
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Then the analysis was repeated using the second primary end-point, which was septic loosening.
In contrast, in our BLSOM for genome analyses, we could obtain a reproducible map using the first and second primary components in the principal component analysis of oligonucleotide composition for the initial vectorial data.
We ran separate models for each outcome of interest, that is, PD, AD, and dementia, using the first available, either primary or secondary, hospitalization for these conditions.
Two negative controls were used; in the first, primary antibody was substituted with antibody diluent, and in the second, an irrelevant rabbit polyclonal antibody was used.
This procedure thereby assured that sorting the ICD-9 codes and using the first code as the primary diagnosis did not omit any other potential primary diagnoses.
The 10 most common primary diagnoses (using the first 3 digits of the ICD-9-CM code) were reported for each age group.
For each encounter, medical problems were encoded using the second version of the International Classification of primary Care (ICPC-2).
If a subject had multiple diagnoses of lung cancer, we used the stage of the first primary diagnosis.
This cell was the first primary battery used for the production of electricity.
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