Sentence examples for using the first cDNA from inspiring English sources

After identification of RNA quality, 2 μg of total RNA from each organ was used to synthesize first-strand cDNA using the first cDNA synthesis Kit (Tiangen, China) in accordance with the manufacturer's instructions (Tiangen, China).

To overcome this problem, we used the first cDNA isolated for MUC4, JER64 (Porchet et al, 1991), to design a mini-MUC4 construct.

RNA samples were retrotranscribed to cDNA using the First Strand cDNA Synthesis Kit (Roche).

RNA was reverse transcribed into cDNA using the First Strand cDNA Synthesis Kit (Fermentas GmbH).

RT-PCR was performed by generating cDNA using the First Strand cDNA Synthesis kit (Invitrogen).

RNA was converted into cDNA using the First Strand cDNA Synthesis kit and oligo dt) primers (Fermentas).

Total RNA (1  μg) was used for the synthesis of first strand cDNA using the First Strand cDNA Synthesis kit (Amersham Pharmacia Biotech, Uppsala, Sweden) following the manufacturer's instructions.

RNA integrity and concentration was analysed using Agilent Technology and 1 μg of RNA was retrotranscribed into cDNA using the First Strand cDNA Synthesis kit from Roche (Mannheim, Germany).

According to the manufacturer's protocol, 0.5  μg of total RNA was converted to cDNA using the First Strand cDNA Synthesis Kit (Fermentas, St.Leon-Rot, Germany).

2  μg total RNA was reverse-transcribed to cDNA, using the first strand cDNA synthesis kit (Qiagen) according to the manufacturer's instructions.

RNA samples (2 μg) were reverse-transcribed to cDNA using the First Strand cDNA Synthase Kit (MBI Fermentas, Hanover, MD) with random hexamer primer.