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Assembled draft genome sequences were compared to their reference genome using the BIGSdb Genome Comparator tool and assessed using the finished genome CDS sequence annotation.
Further, the genomes were annotated in the Kodon software (Applied Maths, Sint Martens-Latem, Belgium) using several publicly available complete C. jejuni genome sequences, and the contigs of the draft sequences were then ordered 1) by aligning all five draft sequences to each other, and 2) by aligning all five genome sequences using the finished genome of C. jejuni 11168 [ 20] as a scaffold.
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The finished genome was annotated using DOGMA (Dual Organellar Genome Annotator) [ 47] and deposited in Genbank [Genbank: KF862711].
This way 154 of originally 223 contigs with a total size of 22 kb were not used which equals less than 0.01% of the finished genome.
The finished genome sequence was achieved by primer walking using a GC sequence finishing kit (GEhealthcare) and transposition bombs.
The finished genome of C. sakazakii SP291 [Genbank accessions CP004091-4] was used as a reference for contig reordering.
The entire chromosomal sequences of the finished genome assembly were partitioned into non-overlapping windows, and their GC levels were calculated using the program draw_chromosome_gc.pl [ 32, 33].
In turn, the mapping of SBS reads on the finished genome sequence revealed no uncovered regions.
The error rate of the finished genome sequence is less than 1 in 100,000.
Polisher was run to correct possible homopolymers and indel errors in the finished genome.
Results of applying PGA using the already finished genome as reference sequence.
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