Finally, parentages which were not confirmed by the GBS results (using the final chosen filtering steps) were assumed unknown.
Importantly, the Swern oxidation was used in the final step to synthesize the nonacene precursor, with a significantly increased reaction yield28.
For blank control, equal volume of the same buffer used in the final step of protein purification was added.
In this study, different mathematical functions were used in the final step of GP modeling, including basic arithmetic operators (left( { +,, -,, times,, div } right)) as well as some other basic mathematical functions (left( {sin,cos,{text{abs}},{text{sqrt}},{text{power}},exp } right)).
Experimental constraints are used in the final step to identify an optimized RNA secondary structure.
Those samples were only used in the final step, at the time of computing the correlation of the gene profile, across all samples, with the Jensen-Shannon divergences computed for the "Control" and "Severe" classes as explained later in this section.
The difference between the equity allocation and the current allocation to each program is the main output used in the final step.
The difference between prescriptive and current priority levels of each program is used in the final step to determine if programs are over- or under-funded.
To concentrate the recovered DNA, 50 μL of elution buffer followed by an additional 50 μL or 75 μL were used in the final step.
Gel filtration (Superdex 200, GE Healthcare, Waukesha, WI) in 25 mM MES (pH 6.0), 100 mM NaCl, and 1 mM EDTA was used for the final step of purification.
