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The mean, standard deviation and relative standard deviation (RSD) of triplicates and the percentage of inhibition were calculated using the final fluorescence intensity measured.
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This study showed that different factors such as temperature, freeze-thawing cycles, substrate concentration, the organic solvent used to dissolve the substrate and the time of incubation of enzyme-substrate mixture influenced the final fluorescence intensity.
The final fluorescence was measured in a Fluoroskan Ascent FL fluorometer (Thermo Fisher Scientific, Waltham, MA).
Fold changes were determined by dividing the final fluorescence by the initial fluorescence.
The final fluorescence level at the proinflammatory state is marginally higher than the fluorescence after 3 μM hypericin).
"Use the final bullet in your gun".
Gray scale images, obtained separately recording Cy3, FluorX and DAPI fluorescence by specific filters, were pseudo colored and merged for the final image using the Adobe Photoshop software.
Tetra-glycine beads showed the most rapid fluorescence increase and the highest final fluorescence.
Place the final boards using the crowbar.
For fluorescence activated cell sorting (FACS) of SW480, the cells were labelled using the same protocol but without the final fixation step.
The bladders were excised after the final MRI scans, re-scanned for whole tissue fluorescence signal using the IVIS system, and then serially sectioned.
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