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The orthologue relationships were determined by best-best hit analyses using the FASTA package [87] on a desk-top computer.
The percentages of similarity were determined using the FASTA network service (European Bioinformatics Institute Fasta Service; available from http://www.ebi.ac.uk/fasta).ac.uk/fasta
Identities were determined by the EBI (European Bioinformatics Institute) using the Fasta 3 network service [ 57].
The fasta and quality files were extracted from the 454 reads (unpaired) in the Standard Flowgram Format SFFfilesles using the python script 'sff_extract.py' and the random first 9-mer and 4 tcag clipped.
The MAF blocks were concatenated and transformed in FASTA file format using the script available in Galaxy [ 35- 37].
Python scripts can also be written and executed within MorphoGraphX using the Python Script process.
Sequence fasta files were processed using the script Trace2dbest [ 74], which incorporated the phred and crossmatch programmes [ 75, 76].
A.fastq-file was produced using the corresponding fasta and quality files of the non-duplicate sequences.
Reads were prepared by converting FASTA sequence and quality files into the CABOG FRG format files using the convert-fasta-to-v2.pl (-454 switch) software [ 19, 20].
Only the loading of one or more FASTA files is mandatory, thereafter zero or more annotation and alignment files may be loaded into GDAV's small MySQL schema using the supplied Perl scripts.
PCR reaction mixtures were prepared using the KAPA SYBR FASTA qPCR kit.
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