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To illustrate the sequence-based search, we first extracted the gene sequences from this bin, by using the FASTA export functionality of chromoWIZ.
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The orthologue relationships were determined by best-best hit analyses using the FASTA package [87] on a desk-top computer.
The percentages of similarity were determined using the FASTA network service (European Bioinformatics Institute Fasta Service; available from http://www.ebi.ac.uk/fasta).ac.uk/fasta
Identities were determined by the EBI (European Bioinformatics Institute) using the Fasta 3 network service [ 57].
The entries are exported using the "NDS field export" function.
Alignments can be exported using the export data link at the left of the view.
P. falciparum exports pathogenicity-related proteins into the host cell using the Plasmodium export element (PEXEL).
A.fastq-file was produced using the corresponding fasta and quality files of the non-duplicate sequences.
Reads were prepared by converting FASTA sequence and quality files into the CABOG FRG format files using the convert-fasta-to-v2.pl (-454 switch) software [ 19, 20].
PCR reaction mixtures were prepared using the KAPA SYBR FASTA qPCR kit.
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