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MiRNA target genes were identified using the FASTA engine of Tapir v1.0 program, with a stringent maximum score of three and minimum free energy ≥ 0.7 [ 84] excluding ESTs annotated as unknown proteins.
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Local alignment of protein sequences was done using the FASTA suite (http://fasta.bioch.virginia.edu/fasta_www2/fasta_down.shtml).shtml
The orthologue relationships were determined by best-best hit analyses using the FASTA package [87] on a desk-top computer.
The percentages of similarity were determined using the FASTA network service (European Bioinformatics Institute Fasta Service; available from http://www.ebi.ac.uk/fasta).ac.uk/fasta
Identities were determined by the EBI (European Bioinformatics Institute) using the Fasta 3 network service [ 57].
It was created using the Ren'Py engine.
Additionally, gray literature was considered using the metasearch engine Google Scholar.
Internet searches were carried out using the specialist engine Intute (www.intute.ac.uk/healthandlifesciences/) and the meta-search engine Copernic (www.copernic.com).
Input of engine characteristics was done using the engine tool.
The MEDLINE database was searched using the OVIDsp search engine.
The medline database was searched using the Pubmed search engine.
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