A 20-μL volume of IC at a predetermined concentration was added to the sample, and DNA was extracted by manual extraction (retrospective study) or automated extraction using the EasyMag extraction system (BioMerieux, Ho Chi Minh City, Vietnam), according to manufacturer's instructions (prospective study).
Zingiber zerumbet powder (2.0 g) was stirred in aqueous ethanol in preparation for extraction using the microwave extraction system.
Nicotine was analyzed by HPLC as described previously (Keinanen et al., 2001) with the following modification of the extraction procedure: approximately 100 mg of frozen tissue was homogenized in 1 mL of extraction buffer using the FastPrep extraction system (Savant Instruments, NY, USA).
After bacterial lysis, DNA was extracted using the EasyMag extraction system (BioMerieux, Ho Chi Minh City, Vietnam), according to manufacturer's instructions and subjected to consecutive monoplex PCR reactions (CSF samples).
The DNA was extracted partly by using the manual extraction system High Pure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany) as previously described [ 14], and partly by the automated evolution of the manual system (MagNA Pure LC DNA extraction kit III on the MagNA Pure LC instrument-Roche Diagnostics, Mannheim, Germany) according to the manufacturer's instructions [ 16].
Amplicons were cleaned using the Gel Extraction System (Viogene, Taipei) and cloned into a pGEM T-Easy vector (Promega, Fitchsburg).
After the user has registered the relevant credentials for the data sources in the SSO infrastructure using a Web Browser interface, the extraction system can access data sources using the central authentication and authorization provided by the Single Sign-On REST interface.
The extraction system used in our study promotes solubility of neutral and basic proteins as evident in the pIs of the identified proteins.
They used the information extraction system called ANNIE, distributed freely with GATE, to preprocess and annotate a training set.
RNA from clinical samples and 25 reference strains (Table 4) was extracted using the automated KingFisher extraction system (Thermo Scientific), MagNA Pure compact or MagNA Pure 96 (Roche Applied Science) instruments according to manufacturer's instructions.
