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The recovery of estradiol using the extraction procedure was 91% [ 11] and all results have been corrected for the extraction loss.
Using the extraction procedure and bioassay, intrinsic Zn (II) concentrations ranging from 1.4 to 4.8 mg/l for milk brands and from 1.2 to 2.9 mg/kg for yoghurt brands were determined, which correlated to those determined using inductively coupled plasma atomic emission spectroscopy.
Concentrations of S-ketamine and S-norketamine were determined using enantioselective capillary electrophoresis (CE) according to the techniques described by Theurillat and colleagues [ 17, 18] using the extraction procedure described in Bergadano et al. [ 19] with phosphoric acid for acidification and reconstitution in 1 1 diluted running buffer without chiral selector.
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Herein, we use the extraction procedure with dithizone and trifluoroacetic acid that was reported by our collaborators, which achieves 92 95% recovery of Zn in serum by stripping voltammetry.
Besides, our results have indicated that, depending on the quantity of the biological samples used, the extraction procedure directly impacted on the G/C composition of the miRNAs detected.
Tables 1, 2, 3, 4, 5 and 6 gives a summary of the plant species that were tested, the family these plants belong to, the parts of the plants that were used to prepare the test samples, the solvent used for the extraction procedure and their potencies in different units depending on the protocol used.
The ligand that was used for the extraction procedure was 1,4,8,11-tetraazacyclotetradecane (cyclam, Merck) ≥92.0 % and used without any further purification, except for vacuum drying over P2O5.
To eliminate possible RNA contamination, RNaseOUT (G-Biosciences, St . Louis MO was used throughout the extraction procedure.
Protease inhibitors (complete Mini EDTA-free, Roche) were used throughout the extraction procedure.
Reagents used in the extraction procedure will be indicated in that section.
The type of solvent used in the extraction procedure determines the success of isolating compounds from plant material [ 29].
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