Data extraction was performed using the Agilent feature extraction software V9.1.3.1.
This result indicated that the extraction-digestion process could not extract all β-casein in dough.
Amplicon was cleaned by digestion with Exonuclease and Shrimp alkaline phosphatase or through gel extraction using the QiaQuick™ Gel Extraction Kit (Qiagen, Valencia, CA).
Furthermore, the extraction efficiency was enhanced using the suspension-digestion process which provided better recoveries compared with the classical protein extraction method.
DNA was extracted either from the entire abdomen using the Chelex® 100 extraction protocol [ 26] or by internal body digestion using the Invisorb extraction kit (Invitek).
DNA was prepared from tissue and blood specimens using the conventional proteinase K digestion and phenol/chloroform extraction method.
Automated extraction also was conducted using the MagNa Pure LC extraction system (Roche).
Ten mg rat hippocampal tissue was used for total RNA extraction using the Trizol method, followed by DNAse digestion.
RNA was isolated via phenol/chloroform extraction using the miRNeasy Kit (Qiagen), performing a DNAse digestion.
Total RNA was extracted from dissected SAM (approximately 80 SAMs per extraction) using the Qiagen RNeasy Mini Kit (Qiagen, Victoria, Australia) with on-column DNAse digestion.
The extraction percentage was calculated using the analyte concentration obtained by a total digestion procedure as a reference.
