The extraction was performed using the extraction tool embedded in the spatial analyst in ArcGIS 10.1.
The mycelia were collected for total RNA extraction using the Plant RNA Extraction Kit (TakaRa MiniBEST).
PCR products and products of restriction digests were purified by gel electrophoresis and extraction using the QIAquick gel extraction kit (Qiagen, Valencia, CA).
Single PCR products were diluted to 30 ng/µl or purified by gel extraction using the QIAquick Gel Extraction kit (Qiagen, Mississauga, ON, Canada), according to manufacturer's instructions and either sent to Canadian Centre of DNA Barcoding, Guelph, ON for DNA sequencing or sequenced directly using BigDye v3.1 reagents on at NAPS unit at University of British Columbia, BC.
Subsequent gel extraction using the QIAquick Gel Extraction Kit (Qiagen) and sequencing (MWG) were performed.
Multiple bands were purified through gel extraction using the QIAquick Gel extraction kit (Qiagen).
We performed gel extraction using the QIAquick Gel Extraction kit and eluted with 35 µL of EB buffer.
The inverse-PCR products were purified by gel extraction using the QIAquick Gel Extraction Kit (QIAGEN) before being sequenced as above.
The specific probes were prepared by digesting the appropriate pBluescript plasmid with XbaI and EcoRI, separating the fragment on a 1.5% agarose gel followed by extraction using the Qiaex II extraction kit (Qiagen).
Interestingly, feature extraction using the Bayes Feature Extraction approach as implemented here is intuitively similar to the application of position-specific scoring matrix (PSSM) profiles for predicting protective linear B-cell epitopes by EL-Manzalawy et al.[ 21].
The blood of animals was collected from the jugular vein and was placed in a tube with acid-citrate-dextrose anticoagulant for genomic DNA extraction using the Tianamp Genomic Extraction Kit (Tiangen, Beijing, China).
