Sentence examples for using the extraction buffer from inspiring English sources

Arabidopsis seedlings or leaves were grounded into powders in liquid nitrogen and total proteins were extracted using the extraction buffer (500 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% NP-40, 4 M urea, 1 mM phenylmethanesulfonyl fluoride).

Total soluble protein was extracted from transformed wheat suspension cells and N. benthamiana leaves using the extraction buffer (20 mM Tris pH 8, 150 mM NaCl, 0.05% TritonX-100, and 100 µM PMSF), separated on 10% SDS-PAGE, and transferred onto PVDF membrane.

Grapes were grinded in liquid nitrogen, seeds removed, and then RNA extraction was carried out using the extraction buffer described by [ 121] with additional 0.8%PVP-40PVP-40

After each step, the samples were centrifuged at 30,000 g and 4°C for 30 min. The supernatants were pooled and subjected to IMAC on Ni-NTA His-bond resin (Novagen) using the extraction buffer as loading and elution buffer, in the latter case supplemented with 500 mM imidazole.

Samples containing 1 × 10 cells were harvested by low speed centrifugation from chronologically aging yeast cultures, washed with cold phosphate buffered saline (PBS), and resuspended to a final OD600 of 10 using the extraction and lysis buffer provided by the fluorescent NADPH/NADP detection kit from Cell Technology Inc. (Mountain View, CA).

Briefly, following treatment cells were harvested and lysed using the Cytosol Extraction Buffer Mix with DTT and Protease Inhibitors, cells were incubated on ice for 10 min prior to being homogenized in a dounce homogenizer.

Total cellular protein was isolated using the protein extraction buffer and determined using the BCS protein assay kit.

After 2-h incubation at 37°C, the optical density (OD) at 450 nm was measured using a 96-well multiscanner autoreader with the extraction buffer used as a blank.

The coffee protein extract was dialysed against the extraction buffer using a Mini Dialysis Kit (1 kDa cut-off) (Amersham Biosciences) to eliminate soluble sugars.

Samples were homogenized in the extraction buffer using the Bullet Blender homogenizer, as mentioned earlier.

Mass spectrometry on extracts of connective tissues is difficult; it is possible, that the extraction buffer used (0.1 M Tris buffer pH 7.5) was inappropriate.