Sentence examples for using the expression vector from inspiring English sources
Recombinant proteins rp(H M180 and the chimeric protein were prepared using the expression vector pQE30 (Qiagen Inc ., expressed in E. coli, isolated, and purified using QIAexpress Ni-NTA Protein Purification System following previously described protocols [23].
The recombinant protein, rPb27, was expressed in E. coli using the expression vector pET-DEST42 (Invitrogen, USA), which produces a recombinant protein with a C-terminal his-tag.
All humanized scFvs were expressed in E. coli periplasm using the expression vector pMD204, specially developed for construction and production of fusion proteins [32].
NFI was expressed in Drosophila S2 Schneider cells using the expression vector pMT-Dest-HisSBP3xV5 (Bonke et al., 2013), protein expression was performed as described previously (Bonke et al., 2013).
Expression of the codon optimized HotAldO gene was only marginally better than the wild-type gene, but by expressing the protein in TOP10 cells using the expression vector pBADHAS, in which the protein was fused to a C-terminal 6xHis tag, approximately 2.5 mg of pure protein could be obtained from 1 L of culture broth using a single Ni2+-NTA agarose chromatography step under native conditions.
We expressed GST-tagged SEpHluorinA277D in E. coli strain BL21 (NEB, USA) using the expression vector pET-42a, which contains a GST-tag and multiple cloning site (Novagen, USA).
The plasmids used for expression of the putative β-glucosidases were constructed using the expression vectors JMP62UraTEF and JMP62LeuTEF, which are derivatives of a previously described vector [ 47].
The 6 × His and GST tags were fused with TAT-Apoptin at the N-terminus using the expression vectors pET28a and pGEX-4 T-1, respectively.
Given a microarray dataset for a cohort S and a subset of samples S0 balanced for relapse, thresholds are determined for the 4 probes in AP4 using the expression vectors restricted to S0.
To vary the Rot content in SA564 we used the expression vector pRMC2 [32].
All of them used the expression vectors to heterologously produce keratinase in B. subtilis.
