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By using the experimental evolution strategy, three l-sorbose-resistant helper strains were isolated.
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This method mimics the conditions used in the experimental evolution setup, but at an individual level.
DOI: http://dx.doi.org/10.7554/eLife.09638.003 10.7554/eLiFigure38.004 Figure 1 figure supplement 1. DIC microscopy image of a microcolony that grew from a single bem1∆ cell that was taken from the colony that was used to start the experimental evolution experiment of the A-lines.
All flies used to constitute the experimental evolution lines were derived from a large outbred wild-type population of D. melanogaster (LHM) that had been maintained under the same rearing protocol for over 400 non-overlapping generations for a detailed description, see [ 49].
The predicted crack growth is in a good agreement with the experimental evolution obtained using Digital Image Correlation (DIC).
ŁM, OYM and MJGG initiated and maintained the experimental evolution lines used in this work.
The experimental evolution is typically used to investigate the adaptation of microbes to specific laboratory conditions (Buckling et al. 2009; Zou et al. 2013).
Although it has certain fortuitousness in obtaining LHS by experimental evolution in a short time, it is enough to confirm the hypothesis that the experimental evolution strategy can be used for LHS screening.
These results suggest that the experimental evolution strategy can be used for LHS screening and Bc 21 is a candidate helper strain for 2-KLG production in high concentration of l-sorbose.
The third part of this study involved the experimental evolution in temporally alternating environments, again using the serial dilution approach.
(A ) Overview of the experimental evolution.
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