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Motif search using the expasy server showed the presence of Walker motif A and B (Fig. S1).
The peptides (minimum 300 Da) were computed using the Expasy server (http://web.expasy.org/peptide_mass/ ).org/peptide_mass/
The pI for each Ig-like domain was computed using the ExPASy server, while electrostatic surfaces were calculated using Delphi.
A transmembrane index was generated for SOD3v2 (SOD3 variant 2) using the ExPASy Server program, ProtScale [ 51], and with UMDHMMTMHP [ 52].
The nucleotide sequence was translated, MW and pI predicted by using the ExPASy server tools [ 50], and multiple-sequence alignments done with ClustalIW v.2 [ 51].
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The theoretical parameters of the deduced protein sequences were computed using the ExPASy Proteomics Server (http://cn.expasy.org/tools/pi_tool.html).html
Protein sequences were obtained by using the ExPASy Proteomics Server tools.
The normalized values of the Kyte & Doolittle hydrophobicity scale for individual residues were obtained using the ExPASy Proteomics Server, http://www.expasy.org/tools/protscale.html.html
Sequence data for the nitrilases were obtained using the ExPASy proteomics server.
Enzyme Mw and pI were predicted using the ExPASy proteomic server program (http://web.expasy.org/compute_pi/).org/compute_pi/
The isoelectric point (pI) and instability index [ 54] of each protein were calculated using the Expasy proteomics server [ 55].
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