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The DNA sequences of the pColE2 ancestral and evolved plasmids were determined by primer walking, using the existing sequence data for the colicin E2 gene cluster as the starting point (Cole et al. 1985).
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The new sequence data as well as the existing sequence data were used to link the genome contigs from Amel_4.0 into more contiguous scaffolds.
Using the existing annotated E. coli tRNA sequences, a total of 73 unique RNase T1 digestion products containing at least one post-transcriptionally modified nucleoside (excluding pseudouridine) are expected.
This family and sub-family classification is based on the amino acid sequence similarity using the existing criteria of less than 40% similarity defining a family and less than 55% similarity defining a sub-family as followed by the International P450 Nomenclature Committee.
The microsatellite loci among the existing sequences were identified by implementing an algorithm into a program (locusfinder.pl) using Perl.
Mishler and his colleagues included in their analysis almost 1,100 lineages representing over 5,000 plant species in the state, using existing sequence data for a specific set of genes taken from DNA databases, and adding sequencing data newly generated at UC Berkeley to fill in gaps.
In addition, it links the mutations to the proteins and genes that appear in text and performs sequence verifications using existing sequence databases to increase the precision of the annotations.
We identified and bioinformatically validated SNPs throughout the rhesus macaque genome using existing sequence data and new SOLiD sequencing reads we generated from three unrelated macaques.
We used the existing annotation (the 'CDS' keywords) to differentiate protein-coding and non-coding sequences.
Hence a more rigorous bioinformatics approach was employed and a monocot-specific C-terminus probe was generated using existing sequence data for MADS-box putative proteins.
Primers were designed using existing sequences for the two species as well as from house cat sequences downloaded from NCBI.
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