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Diversity statistics were calculated according to [ 45] using the Excel microsatellite toolkit [ 46].
Allelic polymorphic information content (PIC) was calculated using the Excel Microsatellite Toolkit 3.3.1 [ 39].
Polymorphism information content (PIC) [ 60] was calculated using the Excel Microsatellite Toolkit [ 61].
For each locus, the number of alleles per locus (A) and the expected heterozygosity (He) were calculated using the Excel Microsatellite Toolkit (Park, 2001).
Microsatellite (autosomal and Z-chromosomal) variation was assessed by the number of alleles and the observed heterozygosity in the males and females using the Excel Microsatellite Toolkit version 3.1.1 [ 54].
Conversion of microsatellite and minisatellite sequences to allelic data was performed by using the Excel Microsatellite Tool Kit 3.1.1 (http://animalgenomics.ucd.ie/sdepark/ms-toolkit/) and Genepop version 4.0 (http://genepop.curtin.edu.au/).edu.au/
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We accepted for further analyses samples that amplified at least 9/14 loci, and used the Excel Microsatellite Toolkit (Park [ 30]) to test for the presence of matching profiles.
Since vegetative propagation is a potential mode of reproduction in S. oppositifolia, we used the Excel Microsatellite Toolkit (http://www.animalgenomics.ucd.ie/sdepark/ms-toolkit/) in each population to distinguish plants with a common clonal ancestry from plants sharing the same microsatellite-based multilocus genotype (MLG) by chance.
Checking of microsatellite data and calculation of expected heterozygosities was performed using the Excel-based microsatellite toolkit (http://animalgenomics.ucd.ie/sdepark/ms-toolkit/).
The problem is resolved using the Excel solver.
Basic statistical analyses were made using the Excel statistical package.
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