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Using the established calibration curve, amounts of H2O2 in RAW264.7 cells stimulated with various amounts of PMA were calculated.
After that, the concentration of H2O2 endogenously produced by PMA-stimulated RAW264.7 cells was determined by measuring the fluorescence intensity, followed by conversion using the established calibration curve.
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Once the optimal parameters were determined for the calibration set, the test set was loaded and class membership probabilities calculated using the established SVM-DA calibration model.
When utilizing the algorithm, working dataset is split into two subsets: a calibration set, which is used to establish the calibration model, and a test set, which is employed to assess the predictive ability of the established calibration model.
The residual dye content was determined by interpolation using the previously established calibration curve.
The cytosolic [Ca2+] was estimated from the ratio measurements using an established calibration method [ 21].
Cells were washed by re-suspension of pellets in mineral salts medium and further diluted to the required cell concentration using previously established calibration curves between optical density measurements and cell numbers as determined by DAPI staining and epifluorescence microscopy for each strain.
The obtained turbidity values and stages at weekly and 5-min time scales were subsequently converted to suspended-sediment concentrations and water discharges, respectively, using established calibration methods.
The latter samples were used to establish calibration curves and served as internal quality control samples during the assay.
7 Calibration was assessed using the calibration slope and a calibration plot.
A nonlinear model of the camera was established, and the calibration of the camera was achieved by using the coplanar calibration method based on the least square method.
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