Sentence examples for using the established TaqMan from inspiring English sources
Maize samples with different contents of genetically modified component were tested using the established TaqMan real-time PCR system.
Three CNV regions— IGKV (immunoglobulin kappa variable cluster at 2p11.2; MIM# 146980), DKK2 (Dickkopf 2 homolog at 4q25; MIM# 605415), and PDZD2 GOLPH3 (PDZ domain containing 2; MIM# 610697; golgi phosphoprotein 3 at 5p13.3; MIM# 612207)—were carried onto the next experimental stage to analyze the full Estonian RM case-control sample set using the established TaqMan qPCR approach (Fig. 1).
Q-PCR was performed and analyzed as described below using the established salmon louse eEF1α standard gene assay [ 64] and the TaqMan assays (FAM) listed in Table 1.
Genotype at position 192 was determined using a previously established TaqMan-based genotyping assay (Applied Biosystems Inc., Foster City, CA) with genomic DNA extracted from EDTA-treated whole-blood specimens (Searles Nielsen et al. 2005).
Relative gene expression levels were determined using the TaqMan Fast Universal PCR Master Mix Applied Biosystemss) and FAM-labeled TaqMan gene expression assays.
Taqman RT-qPCR was performed to detect miRNA expression levels using the Taqman miRNA Reverse Transcription kit and Taqman universal PCR Master Mix Applied Biosystemss).
mRNA qRT-PCR was performed using the TaqMan High-Capacity cDNA Reverse Transcription Kit, TaqMan Fast PCR Master Mix, and TaqMan mRNA assay primers (Applied Biosystems).
Primers and TaqMan probes [see Additional File 1] have been designed using the proprietary TaqMan software.
qRT-PCR was performed on a Agilent Mx3005P using the TaqMan Universal PCR Master Mix Applied Biosystemss part number 4304437) and the TaqMan probe from the respective TaqMan MicroRNA Assay kit.
In brief, DNA samples were mixed with the primers and the TaqMan probe and amplified using the TaqMan Fast Universal PCR Master Mix Applied Biosystemss).
TaqMan assays were used to quantify SHP-1 (Mm00469153_m1) expression using the TaqMan Gene Expression Master Mix (Applied Biosystems).
