Sentence examples for using the est database from inspiring English sources

However, because so many of the ESTs in the EST database proved to be contaminated by either vector or sequencing primers (an unsuspected impediment to others using the EST database), we cleaned the matching 2429 ESTs and re-ran the analysis to yield 1782 validated EST alignments (http://bioserv.pbf.hr/Supplementary_Data/File2) for annotation.

Using the EST database and chicken data, possible alternative splicing was detected for six genes.

However, with stringent searches using the EST database, more TPX2 gene sequences were identified also in the Gymnosperms.

The putative full-length or partial open reading frames of PMCAs and NCXs obtained were confirmed using the EST database, and/or were used to design primers for cloning and the RT-PCR analysis.

So far, we were able to identify one representative of the CLE family from Physcomitrella patens using the EST database, although more might be found once the genome of this organism is made publicly available.

EST-SSR (simple sequence repeats in expressed sequence tags) markers are useful in these studies, because highly polymorphic markers can be developed with relative ease using the EST database (e.g., Sakaguchi et al., 2011) and they are less susceptible to null alleles than anonymous SSRs (Ellis and Burke, 2007).

However using the capsicum EST database, one EST closely matched to LeETR3 (named CaETR3) and two ESTs related to LeETR4 and LeETR5 (named CaETR4 and CaETR5, respectively) were identified (all partial).

After the clones whose transcription was conspicuously up-regulated in PBMCs as compared with TIG-1 cells were sequenced, the DNA sequences were used to search the EST database by using the BLAST algorithm (http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/

Fruit and flower samples from the major Actinidia varieties that were used to produce the EST database were characterized using both headspace sampling and solvent extraction followed by GC-FID/MS analysis.

The identification of conserved miRNAs by searching all available nucleotide databases allows for wider and better coverage of diverse plant species than that with use of the EST database alone.

To determine if the identified binding site was near a gene, 6 kb of genomic DNA (3 kb on each side) was used to BLAST against the EST database using blastn.