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A 500 bp HT-A probe was synthesized using the entire coding region of a ScHT-A1 cDNA from S. chacoense [32].
For this reason, the analysis was done twice: Once using the entire coding regions and again with any edited codons and the six preceding codons removed from the alignment.
Expressed match was either identified through TblastN search against EST resources using MHCI alpha 3 domains or when this approach was negative expressed match was sought using the entire coding sequence in GenBank nucleotide (cDNA) and subsequently available TSA/SRA resources.
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pAA, pNT, pS, and pN, represent average number of amino acid, nucleotide, synonymous and non-synonymous nucleotide differences per site when calculated using the entire protein/nucleotide coding sequence along with the Z-test of selection.
Next, interviews 1 3 were recoded for second-level codes and then coded again using the entire list of new codes.
ThIn casebe attributed tofthe faCHAUHandand KAM always try to assign a higher-rate code when the OVSF code tree hanyenough codecity, unlike CFCassignmentisns a hinformedte code only when the valusing CRD becomes greathe than 0. Figurentireobranchty of delandviolayernumbersus lofd.
In our study, we elected to limit false negatives and identify more potential lineage-specific genes in A. thaliana by using the entire A. thaliana predicted protein-coding gene complement in our analyses and by using more stringent searching criteria.
Other tools such as error checking, both at the time of coding and later using the entire data set of the hospitals, also offer opportunities for improving coding accuracy beyond that afforded by external audits.
MLPA assay was used for screening the entire coding region.
6 pairs of genome specific primers were used to span the entire coding sequence of Ntl.
The set of overlapping primers selected was used to cover the entire coding sequence of the gene.
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