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B. pseudomallei was isolated from one specimen (0.4%) using standard media and from two specimens (0.8%) using the enhanced protocol.
Two specimens (0.8%) were positive for B. pseudomallei using the enhanced protocol whereas one specimen (0.4%) was positive using standard methods.
Two hundred and forty one sputum specimens deemed acceptable for culture were received by the laboratory during the study period and tested using the enhanced protocol.
In September 2007, a pilot study to assess the feasibility of using the enhanced protocol was undertaken in Tartu based on 30 forensic autopsies of men aged 40-54 years. 40-54 years
The single specimen positive using standard media was also positive using the enhanced protocol; the enhanced technique therefore detected one additional isolate of B. pseudomallei compared to the standard protocol.
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The membranes were incubated with anti-rabbit horseradish peroxidase-linked secondary antibody (diluted 1/10 000; Amersham) in 1% milk/PBS/0.05% Tween 20 and results were visualized using the enhanced chemiluminescence protocol (Amersham).
PerDiem at the standard 1 64 use-dilution was used for the enhanced protocol.
In the proposed mechanism, the cluster-head selection is implemented using the enhanced hybrid wireless mesh protocol (E-HWMP) protocol, which is based on the IEEE802.11p and IEEE802.11s standards.
In this study we aimed to determine whether an intraoperative optimization protocol using the enhanced flow-based hemodynamic parameters of the FloTrac/Vigileo device would result in an improvement in outcome in high-risk patients undergoing major abdominal surgery, measured by the length of hospital stay (LOS) compared with a standard protocol based on conventional hemodynamic data.
Immunoreactive bands were visualized using the enhanced chemiluminescence detection system (Santa Cruz Biotechnology) following the protocol of the manufacturer.
Immunodetection was performed using the enhanced chemiluminescent (ECL) kit (GE Life Sciences) according to the manufacturer's protocol and a gel documentation system (Alpha Innotech Corporation).
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