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The discrete-die concept involves using the ends of densely packed rod-shaped elements as the working surface of the die.
So at present we can't rule out the existence of a special packet of 20 cigarettes thoughtfully arranged so that each touches all of the others without using the ends of the cigarettes to make contact.
Second, there is a tendency to avoid using the ends of the scales in subjective judgements where responders have some doubts about "proper" answers [ 33].
We first performed autocorrelation analysis using the ends of H4S47C-anchored cleavage fragments to see if there is any periodicity in centromeric nucleosome positions despite the overall smooth distribution of fragment lengths.
As separate de novo assembly (of B1K2 and B1K30) was performed using the following parameters: -cdna (to assemble transcripts), -ml 90% (minimum overlap read length, default 40 bp), -urt (to extend contigs using the ends of single read) and -v (a fasta file database for trimming of the contaminants: adapters, primers and poly-A/T).
Position them using the ends of the wires.
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After fragmentation, the fragments were blunt-ended and phosophorylated at the 5' end using the End-it Kit (Epicentre) according to the manufacturer's instructions.
Auden, in "The Orators", wrote a sestina using the end-words country, vats, wood, bay, clock and love.
using the End-iT DNA End-Repair Kit (Epicentre, Madison WI).
Briefly, DNA ends were polished using the End-It kit (Epicentre) and then purified using MinElute reaction cleanup column (Qiagen).
The sheared DNA was immediately blunt end-repaired at room temperature for 45 min using the End-It end-repair kit (Epicentre, Madison, WI).
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CEO of Professional Science Editing for Scientists @ prosciediting.com