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Both genes were successfully integrated into the H. pluvialis genome, either separately or together, using the endogenous norflurazon resistant mutated gene of phytoene desaturase as a selection marker.
This article describes the design and synthesis of AR-C118925, a potent and selective non-nucleotide antagonist of the P2Y2 receptor discovered using the endogenous P2Y2R agonist UTP as the chemical starting point.
However, using the endogenous processing machinery, optimized shRNA constructs allow for high potency and sustainable effects using low copy numbers resulting in less off-target effects, particularly if embedded in a miRNA scaffold.
Using the endogenous human VEGF-A gene, we demonstrated that the new expression system amplified ATF expression in a manner dependent on the number of copies of the ATF-binding site, and that the 'self-propagating ATF' upregulated VEGF-A gene expression more efficiently than a control promoter with no ATF-binding site.
Cell proliferation was also studied using the endogenous marker, KI-67.
Wild-type and mutant PKA proteins were expressed using the endogenous TPK1 promoter in LEU2-marked plasmids.
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The relatively high expression level of miR-19 in HEK293 cells allowed us to use the endogenous miR-19 to inhibit 19RE reporter expression.
Seamless ligation cloning extract (SLiCE) cloning uses the endogenous recombination activity of E. coli cellular extracts in vitro to ligate insert and vector DNA fragments.
Use the endogenous polarity chunks to perform a maximum forward matching segmentation on the given Plurk training dataset, looking for chunks with polarities of higher confidence levels and of the longest length first.
We use the endogenous cross-section distribution to compute several inequality indexes and we also assess the aggregate welfare intensity measured as a percentage change of life-time consumption.
The transgene uses the endogenous procyclin/GPEET promoter and a 3'UTR derived from the aldolase mRNA.
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